The results showed that the open reading frame of chil - 15 cdna encompassed 564 base pairs ( bp ) and encoded a protein of 187 amino acids with three potential n - linked glycosylation sites , four conserved cysteine residues , two out - of - frame atg initiation codons in the 5 " untranslated region , and a signal peptide consisting of 66 amino acids . when it was compared with the published sequence of chil - 15 cdna , 7 mutant sites were found , and 5 amino acids were changed in predicted amino acids , which indicated that chil - 15 may be polymorphic 結(jié)果顯示,本研究所用白來航雞il - 15cdna5 ’非編碼區(qū)有兩個框外atg起始密碼子,開放閱讀框由564bp組成,編碼187個氨基酸,其中n末端信號肽含有66個氨基酸殘基,在第48 、 149和166位的天冬酰胺殘基上有三個潛在的n -糖基化位點。
In the field of biochemistry, N-linked glycosylation is the attachment of a sugar molecule (a process known as glycosylation) to a nitrogen atom in an amino acid residue in a protein. This type of linkage is important for both the structure and function of some eukaryotic proteins.